The motion was submitted at 1pm at the office of Speaker Tan Sri Pandikar Amin Mulia.
Although the motion was accepted by Pandikar's office, a decision has not been made on whether it will be included in the order paper for the current sitting which will be discussing Budget 2016.
A total of 112 votes from the 222 parliamentarians are needed for the motion to be passed.
Even with the support of PAS, the Opposition can only garner 88 votes. Thus, the final decision on the motion rests on Barisan National MPs.
"You have to wait and see," said Dr Wan Azizah, who is PKR president, said at Parliament lobby when asked if any government MPs would support the motion.
She added that Pandikar received the motion even though he earlier suggested that the opposition submit names of those who would back the motion.
"It must be noted that the motion only relates to the loss of confidence in Datuk Seri Najib but not in the Government.
"This is because I and many others irrespective of political affiliations believe that the difficult economic, social and political crisis this country is facing is due to the leadership crisis in the country," Dr Wan Azizah said.
"I trust that all Members of Parliament would vote according to their conscience. We must put aside our politics and put the interest of this nation above all else. We cannot keep someone in office and have thousands lose their jobs," she said.
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